Sandwich ELISA with human plasma using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
Sandwich ELISA with human IgG3 using recombinant Human IgG3 antibody as the capture, and biotinylated anti-human light chains ( kappa;+ lambda;) antibody RM129 as the detect, followed by an AP conjugated streptavidin.
IHC testing of FFPE human lymphoid tissue with recombinant Human IgG3 antibody. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
ELISA of human immunoglobulins shows recombinant Human IgG3 antibody reacts only to hIgG3. No cross reactivity with IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse/rat/goat IgG.
ELISA Titration: the plate was coated with different amounts of hIgG3. A serial dilution of recombinant Human IgG3 antibody was used as the primary and an alkaline phosphatase conjugated anti-rabbit IgG as the secondary.
Supplier Page from NSJ Bioreagents for Human IgG3 Antibody [Recombinant Rabbit Monoclonal]